Genetic Analysis and Molecular Mapping of Light-Sensitive Red-Root Mutant in Rice

doi:10.1016/S1672-6308(08)60053-0

Abstract

Abstract: The light-sensitive red-root mutant, designated as HG1, was newly observed from an indica rice variety, Nankinkodo, when seedlings were grown with roots exposed to natural light. The root color of the mutant began to turn slight-red when the roots were exposed to the light at the intensity of 29 μmol/(m²·s), then turned dark-red at the light intensity of 180 μmol/(m²·s), suggesting that the root color of the mutant was evidently sensitive to light. Furthermore, genetic analysis showed that the character of light-sensitive red-root of the HG1 mutant was controlled by a single dominant gene, tentatively designated as Lsr. With simple sequence repeat markers, Lsr gene was located between the markers RM252 and RM303 on chromosome 4 with the genetic distances of 9.8 cM and 6.4 cM, respectively. These results could be useful for fine mapping and cloning of Lsr gene in rice.

Key words: rice, light sensitivity, red root mutant, genetic analysis, gene mapping

Zhang Jun-zhi, Liu Xiao, Li Chao, Xiao Ke, Dong Yan-jun. Genetic Analysis and Molecular Mapping of Light-Sensitive Red-Root Mutant in Rice[J]. RICE SCIENCE, 2009, 16(1): 27-32 .

References

1 Wu J, Cheng J H, Yang F C. Transcriptional regulation of anthocyanin biosynthesis in plants. Chinese J Cell Biol, 2006, 28: 453–456. (in Chinese with English abstract)
2 Tang C H. Plant Bioactive Substances. Beijing: Chemical Industry Publishing House, 2004: 218–231. (in Chinese)
3 Acquaviva R, Russo A, Galvano F, Galvano G, Barcellona M L, LiVolti G. Cyanidin and cyaniding 3-O-beta-D-glucoside as DNA cleavage protectors and antioxidants. Cell Biol Toxicol, 2003, 19(4): 243–252.
4 Lazzé M C, Pizzala R, Savio M, Stivala L A, Prosperi E, Bianchi L. Anthocyanins protect against DNA damage induced by tert-butyl-hydroperoxide in rat smooth and hepatoma cells. Mutat Res, 2003, 535(1): 103–115.
5 Russo A, La F L, Acquaviva R, Campisi A, Raciti G, Scifo C. Ochratoxin A-induced DNA damage in human fibroblast: protective effect of cyaniding 3-O-beta-D-glucoside. J Nutr Biochem, 2005, 16(1): 30–37.
6 Chung H S, Woo W S. A quinoline alkaloid with antioxidant activity from the aleurone layer of anthocyanin-pigmented rice. J Nat Prod, 2001, 64(12): 1579–1580.
7 Hu P S. Research and utilization of functional nutritional rice. China Rice, 2003(5): 3–5. (in Chinese)
8 Zhang M W, Guo B J. Summary on nutritional features and health-care function of pigmented rice grain and its processing techniques. Acta Agric Shanghai, 2002, 18(suppl): 18–24. (in Chinese with English abstract)
9 Hadagal B N, Manjunath A, Gouo V J. Linkage of genes for anthocyanin pigmentation in rice (Oryza sativa L.). Euphytica, 1980, 30: 747–754.
10 Wataru S, Taku O, Keisuke K, Chikara M, Akira S, Minoru M, Kazuhiko N, Masahiko M. The purple leaf locus of rice: The plw allele has a complex organization and includes two genes encoding basic helix-loop-helix proteins involved in anthocyanin biosynthesis. Plant Cell Physiol, 2001, 42(9): 982–991.
11 Sun M M, Han L Z, Li G X, Hong X T, Yu Y J. Advances in genetic research of gain anthocyanins content in rice. J Plant Genet Res, 2006, 7(2): 239–245. (in Chinese with English abstract)
12 Harbome J B, Grayer R J. The anthocyanins. In: Harborne J B. The Flavonoids. London: Chapman and Hall, 1988: 1–20.
13 Thakur M, Nozzolillo C. Anthocyanin pigmentation in roots of Impatiens species. Can J Bot, 1978, 56: 2898–2903.
14 Stenlid G. On the effects of some flavonoid pigments upon growth and ion absorption of wheat roots. Physiol Plant, 1961, 14: 659–670.
15 Ishikura N, Hayashi K. Anthocyanins in red roots of a radish: Studies on anthocyanins. XXXVII. Bot Mag Tokyo, 1962, 75: 28–36.
16 Duke S O, Naylor A W. Light control of anthocyanin biosynthesis in Zea seedlings. Physiol Plant, 1976, 37: 6248.
17 Solangaarachchi S M, Kevins G. Anthocyanin pigmentation in the adventitious roots of Metrosideros excelsa (Myrtaceae). New Zealand J Bot, 2001, 39: 161–166.
18 Wu S Q, Min S T, Li X S, Deng W H, Study on determination method of anthocyanin of black rice. Amino Acids & Biotic Resour, 2001, 23(4): 44–66. (in Chinese with English abstract)
19 McCouch S R, Kotcket G, Yu Z H, Wang Z Y, Khush G S, Coffman W R, Tanksley S D. Molecular mapping of rice chromosome. Theor Appl Genet, 1988, 76: 815–829.
20 Lincoln S E, Daly M J, Lander E S. Constructing genetic linkage maps with MAPMAKER/Exp 3.0. 3rd edn. Whitehead Institute Technical Report. Cambridge MA: Whitehead Institute, 1992.
21 Reddy A R. Genetic and molecular analysis of the anthocyanin pigmentation pathway in rice. In: Khush G S. Rice Genetics III. Proceedings of the Third International Rice Genetics Symposium, 16–20 Oct 1995. Manila, Philippines: International Rice Research Institute, 1996: 341–352.
22 Fan F J, Fan Y Y, Du J H, Zhuang J Y. Fine mapping of C (chromogen for anthocyanin) gene in rice. Rice Sci, 2008, 15(1): 1-6.
23 Singh A P, Prasad S C, Tomar J B, Paul A. Expressivity of pleiotropic genes governing anthocyanin pigmentation in male sterile line of rice. J Res Birsa Agric Univ, 1997, 9(2): 123–127.
24 Zhuang J Y, Yang C D, Qian H R, Zhao C Z, Zheng K L. Linkage analysis of RFLP markers and the gene for purple. Acta Genet Sin, 1996, 23(5): 372–375. (in Chinese with English abstract)
25 Phoka N, Tragonrung S, Vanavichit A. Anthocyanin intensity in rice grain is regulated by splicing efficiency of dihydroflavono1-4-reductase and is temperature sensitive. [2008-02-28]. http://dna.kps.ku.ac.th/rice_files/rice for future/ poster_rice_anthocyanin.htm.

[1]	Prathap V, Suresh KUMAR, Nand Lal MEENA, Chirag MAHESHWARI, Monika DALAL, Aruna TYAGI. Phosphorus Starvation Tolerance in Rice Through a Combined Physiological, Biochemical and Proteome Analysis [J]. Rice Science, 2023, 30(6): 8-.
[2]	Serena REGGI, Elisabetta ONELLI, Alessandra MOSCATELLI, Nadia STROPPA, Matteo Dell’ANNO, Kiril PERFANOV, Luciana ROSSI. Seed-Specific Expression of Apolipoprotein A-I_Milano Dimer in Rice Engineered Lines [J]. Rice Science, 2023, 30(6): 6-.
[3]	Sundus ZAFAR, XU Jianlong. Recent Advances to Enhance Nutritional Quality of Rice [J]. Rice Science, 2023, 30(6): 4-.
[4]	Kankunlanach KHAMPUANG, Nanthana CHAIWONG, Atilla YAZICI, Baris DEMIRER, Ismail CAKMAK, Chanakan PROM-U-THAI. Effect of Sulfur Fertilization on Productivity and Grain Zinc Yield of Rice Grown under Low and Adequate Soil Zinc Applications [J]. Rice Science, 2023, 30(6): 9-.
[5]	FAN Fengfeng, CAI Meng, LUO Xiong, LIU Manman, YUAN Huanran, CHENG Mingxing, Ayaz AHMAD, LI Nengwu, LI Shaoqing. Novel QTLs from Wild Rice Oryza longistaminata Confer Rice Strong Tolerance to High Temperature at Seedling Stage [J]. Rice Science, 2023, 30(6): 14-.
[6]	LIN Shaodan, YAO Yue, LI Jiayi, LI Xiaobin, MA Jie, WENG Haiyong, CHENG Zuxin, YE Dapeng. Application of UAV-Based Imaging and Deep Learning in Assessment of Rice Blast Resistance [J]. Rice Science, 2023, 30(6): 10-.
[7]	Md. Forshed DEWAN, Md. AHIDUZZAMAN, Md. Nahidul ISLAM, Habibul Bari SHOZIB. Potential Benefits of Bioactive Compounds of Traditional Rice Grown in South and South-East Asia: A Review [J]. Rice Science, 2023, 30(6): 5-.
[8]	Raja CHAKRABORTY, Pratap KALITA, Saikat SEN. Phenolic Profile, Antioxidant, Antihyperlipidemic and Cardiac Risk Preventive Effect of Chakhao Poireiton (A Pigmented Black Rice) in High-Fat High-Sugar induced Rats [J]. Rice Science, 2023, 30(6): 11-.
[9]	LI Qianlong, FENG Qi, WANG Heqin, KANG Yunhai, ZHANG Conghe, DU Ming, ZHANG Yunhu, WANG Hui, CHEN Jinjie, HAN Bin, FANG Yu, WANG Ahong. Genome-Wide Dissection of Quan 9311A Breeding Process and Application Advantages [J]. Rice Science, 2023, 30(6): 7-.
[10]	JI Dongling, XIAO Wenhui, SUN Zhiwei, LIU Lijun, GU Junfei, ZHANG Hao, Tom Matthew HARRISON, LIU Ke, WANG Zhiqin, WANG Weilu, YANG Jianchang. Translocation and Distribution of Carbon-Nitrogen in Relation to Rice Yield and Grain Quality as Affected by High Temperature at Early Panicle Initiation Stage [J]. Rice Science, 2023, 30(6): 12-.
[11]	Nazaratul Ashifa Abdullah Salim, Norlida Mat Daud, Julieta Griboff, Abdul Rahim Harun. Elemental Assessments in Paddy Soil for Geographical Traceability of Rice from Peninsular Malaysia [J]. Rice Science, 2023, 30(5): 486-498.
[12]	Monica Ruffini Castiglione, Stefania Bottega, Carlo Sorce, Carmelina SpanÒ. Effects of Zinc Oxide Particles with Different Sizes on Root Development in Oryza sativa [J]. Rice Science, 2023, 30(5): 449-458.
[13]	Tan Jingyi, Zhang Xiaobo, Shang Huihui, Li Panpan, Wang Zhonghao, Liao Xinwei, Xu Xia, Yang Shihua, Gong Junyi, Wu Jianli. ORYZA SATIVA SPOTTED-LEAF 41 (OsSPL41) Negatively Regulates Plant Immunity in Rice [J]. Rice Science, 2023, 30(5): 426-436.
[14]	Ammara Latif, Sun Ying, Pu Cuixia, Noman Ali. Rice Curled Its Leaves Either Adaxially or Abaxially to Combat Drought Stress [J]. Rice Science, 2023, 30(5): 405-416.
[15]	Liu Qiao, Qiu Linlin, Hua Yangguang, Li Jing, Pang Bo, Zhai Yufeng, Wang Dekai. LHD3 Encoding a J-Domain Protein Controls Heading Date in Rice [J]. Rice Science, 2023, 30(5): 437-448.